STUDY ON PREPARATION AND CHARACTERIZATION OF SACHI SEED OIL BASED EMULSION CONTAINING COLLAGEN PEPTIDES

Authors

  • Nguyen Thuy Chinh Institute for Tropical Technology, Vietnam Academy of Science and Technology, Hanoi city, Vietnam
  • Cung Dinh Duc Faculty of Chemical Technology, Hanoi University of Industry, Hanoi city, Vietnam
  • Nguyen Ngoc Quynh Faculty of Chemical Technology, Hanoi University of Industry, Hanoi city, Vietnam
  • Thai Hoang Institute for Tropical Technology, Vietnam Academy of Science and Technology, Hanoi city, Vietnam
  • Nguyen Phi Hung Institute of Chemistry, Vietnam Academy of Science and Technology, Hanoi city, Vietnam
  • Pham Thi Thu Trang Faculty of Chemistry, Vietnam Military Medical University, Hanoi city, Vietnam
  • Vu Quoc Trung Faculty of Chemistry, Hanoi National University of Education, Hanoi city, Vietnam

DOI:

https://doi.org/10.18173/2354-1059.2024-0024

Keywords:

collagen peptides, sachi seed oil, emulsion, stability

Abstract

In this study, collagen peptides were carried by a Sacha inchi (sachi) seed oil-based emulsion with Tween 80 under optimal conditions: 26.26% sachi seed oil, 26.06% Tween 80, and 47.68% distilled water. Collagen peptides, varying from 5 - 30% of the total emulsion mass, were dispersed in the aqueous phase before being introduced into the emulsion. Using the sachi seed oil-based emulsion to carry collagen peptides helps prevent the hydrolysis of collagen peptides by acids and enzymes in the digestive system. The characteristics of the emulsion were evaluated using dynamic light scattering, infrared spectroscopy, and ultraviolet-visible spectroscopy. The emulsions loaded with collagen peptides have average droplet sizes ranging from 83.57 - 286.93 nm. Emulsions carrying 5 - 10% collagen peptides exhibit a high stability for up to 30 days. These results demonstrate the potential application of sachi seed oil emulsions carrying collagen peptides in functional foods and healthcare products.

References

[1] Subhan F, Hussain Z, Tauseef I, Shehzad A & Wahid F, (2021). A review of recent advances and applications of fish collagen. Critical Reviews in Food Science and Nutrition, 61(6), 1027-1037. https://doi.org/10.1080/10408398.2020.1751585.
[2] Wang Y, Wang Z & Dong Y, (2023). Collagen-based biomaterials for tissue engineering. ACS Biomaterials Science & Engineering, 9(3), 1132-1150. https://doi.org/10.1021/ acsbiomaterials.2c00730.
[3] Rezvani GE, Nourbakhsh N, Akbari KM, Zare M & Ramakrishna S, (2021). Collagen-based biomaterials for biomedical applications. Journal of Biomedical Materials Research B Appl Biomater, 109(12), 1986-1999. https://doi.org/10.1002/ jbm.b.34881.
[4] Gaikwad S & Kim MJ, (2024). Fish by-product collagen extraction using different methods and their application. Marine Drugs, 22(2), 60. https://doi.org/10.3390/ md22020060.
[5] Sarkar MSI, Hasan MM, Hossain MS, Khan M, Islam AA, Paul SK, Rasul MG & Kamal M, (2023). Exploring fish in a new way: A review on non-food industrial applications of fish. Heliyon, 9(12), e22673. https://doi.org/10.1016/j.heliyon. 2023.e22673.
[6] Tran TM, Nguyen TC, Vu QM, Nguyen TTT, Tran DMT, Vu QT, Ha VH & Thai H, (2019). Effect of fish scale collagen on some characteristics and drug release of carrageenan/collagen/allopurinol film. Vietnam Journal of Science and Technology, 57 (3B), 1-8. http://dx.doi.org/10.15625/2525-2518/57/3B/14044.
[7] Watanabe KM, Shimizu M, Kamiyama S, Taguchi T, Sone H, Morimatsu F, Shirakawa H, Furukawa Y & Komai M, (2010). Absorption and effectiveness of orally administered low molecular weight collagen hydrolysate in rats. Journal of Agricultural and Food Chemistry, 58, 835-841. https://doi.org/10.1021/jf9031487.
[8] Liu D, Liang L, Regenstein JM & Zhou P, (2012). Extraction and characterization of pepsin-solubilised collagen from fins, scales, skins, bones, and swim bladders of bighead carp (Hypophthalmichthysnobilis). Food Chemistry, 133, 1441-1448. https://doi.org/10.1016/j.foodchem.2012.02.032.
[9] Ding J, Li Y, Xu J, Su X, Gao X & Yue F, (2011). Study on the effect of jellyfish collagen hydrolysate on anti-fatigue and anti-oxidation. Food Hydrocolloids l, 25, 1350-1353. https://doi.org/10.1016/j.foodhyd.2010.12.013.
[10] Zhang Z, Wang J, Ding Y, Dai X & Li Y, (2011). Oral administration of marine collagen peptides from Chum Salmon skin enhances cutaneous wound healing and angiogenesis in rats. Journal of the Science of Food and Agriculture, 91(12), 2173-2179. https://doi.org/10.1002/jsfa.4435.
[11] Yamada S, Yamamoto K, Nakazono A, Matsuura T & Yoshimura A, (2021). Functional roles of fish collagen peptides on bone regeneration. Dental Materials Journal, 40(6), 1295-1302. https://doi.org/10.4012/dmj.2020-446.
[12] Hou NT & Chen BH, (2023). Preparation of nanoemulsions with low-molecular-weight collagen peptides from sturgeon fish skin and evaluation of anti-diabetic and wound-healing effects in mice. Pharmaceutics, 15(9), 2304. https://doi.org/10.3390/ pharmaceutics15092304.
[13] Kupper S, Chomiczewska IK & Szumała P, (2017). Collagen and hyaluronic acid hydrogel in water-in-oil microemulsion delivery systems. Carbohydrate Polymers, 175, 347-354. https://doi.org/10.1016/j.carbpol.2017.08.010.
[14] Shukla T, Upmanyu N, Agrawal M, Saraf S, Saraf S & Alexander A, (2018). Biomedical applications of microemulsion through dermal and transdermal route. Biomedicine & Pharmacotherapy, 108, 1477-1494. https://doi.org/10.1016/j.biopha. 2018.10.021.
[15] Magrode N, Poomanee W, Kiattisin K & Ampasavate C, (2024). Microemulsions and nanoemulsions for topical delivery of tripeptide-3: from design of experiment to anti-sebum efficacy on facial skin. Pharmaceutics, 16 (4), 554. https://doi.org/10.3390% 2Fpharmaceutics16040554.
[16] Nguyen TC & Thai H, (2023). Review: emulsion techniques for producing polymer based drug delivery systems. Vietnam Journal of Science and Technology, 61 (1), 1-26. https://doi.org/10.15625/2525-2518/17666.
[17] Nur ARMR & Lai KL, (2022). Sacha Inchi (Plukenetia Volubilis L.): recent insight on phytochemistry, pharmacology, organoleptic, safety and toxicity perspectives. Heliyon, 8 (9), e10572. https://doi.org/10.1016/j.heliyon.2022.e10572.
[18] Alexandra V, Frank LRO, Adriana VO, Dayana BR, Ana MM & Fernando RE, (2021). Sacha Inchi Seed (Plukenetia volubilis L.) Oil: Terpenoids, Terpenes and Terpenoids - Recent Advances, (Eds) Shagufta P and Areej MAT, Intechopen, London, United Kingdom.
[19] Ankit G, Beenu T, Manvesh KS & Vivek S, (2022). Sacha inchi (Plukenetia volubilis L.): An emerging source of nutrients, omega-3 fatty acid, and phytochemicals. Food Chemistry, 373 (B), 131459. https://doi.org/10.1016/j.foodchem.2021.131459.
[20] Norhazlindah MF, Jahurul MHA, Norliza M, Shihabul A, Shahidul I, Nyam KL & Zaidul ISM, (2023). Techniques for extraction, characterization, and application of oil from sacha inchi (Plukenetia volubilis L.) seed: a review. Journal of Food Measurement and Characterization, 17, 904–915. https://doi.org/10.1007/s11694-022-01663-0.
[21] Sunan W, Fan Z & Yukio K, (2018). Sacha inchi (Plukenetia volubilis L.): Nutritional composition, biological activity, and uses. Food Chemistry, 265, 316-328. https://doi.org/10.1016/j.foodchem.2018.05.055.
[22] Muangrat R, Veeraphong P & Chantee N, (2018). Screw press extraction of Sacha inchi seeds: Oil yield and its chemical composition and antioxidant properties. Journal of Food Processing and Preservation, 42(6), e13635. https://doi.org/ 10.1111/jfpp.13635.
[23] Mai HC, Nguyen DC, Nguyen PTN & Bach LG, (2020). Physico-chemical properties of sacha inchi (Plukenetia volubilis L.) seed oil from Vietnam. Asian Journal of Chemistry, 32, 335-338. https://doi.org/10.14233/ajchem.2020.22233.
[24] Chirinos R, Zuloeta G, Pedreschi R, Mignolet E, Larondelle Y & Campos D, (2013). Sacha inchi (Plukenetia volubilis): A seed source of polyunsaturated fatty acids, tocopherols, phytosterols, phenolic compounds, and antioxidant capacity. Food Chemistry, 141 (3), 1732-1739. https://doi.org/10.1016/j.foodchem.2013.04.078.
[25] Dam XT, Cung DD, Nguyen NQ, Pham TT, Thai H, Nguyen PH & Nguyen TC, (2024). Optimizing the preparation of nanoemulsions based on sacha inchi (Plukentia volubilis L.) seed oil by response surface methodology. Vietnam Journal of Science and Technology, in press.
[26] Vu TTL, Vu TNL, Mai DH, Nguyen XT, Nguyen PH, Ngo TCQ, Dam XT &Thai H, (2024). Optimizing preparation of fish scale collagen peptide/sacha inchi (Plukenetia volubilis L.) seed oil nanoemulsion. ChemistrySelect, 9 (11), e202303659. https://doi.org/10.1002/slct.202303659.
[27] More SK & Pawar AP, (2020). Preparation, optimization, and preliminary pharmacokinetic study of curcumin encapsulated turmeric oil microemulsion in zebrafish. European Journal of Pharmaceutical Sciences, 155, 105539. https://doi.org/10.1016/j.ejps. 2020.105539.
[28] Yeon JJ, Petra KH & Schaaf US, (2019). Collagen peptide-loaded W1/O single emulsions and W1/O/W2 double emulsions: influence of collagen peptide and salt concentration, dispersed phase fraction and type of hydrophilic emulsifier on droplet stability and encapsulation efficiency. Food & Function, 10, 3312. https://doi.org/10.1039/C8FO02467G.
[29] Bui VH, Nguyen TAS & Tran KV, (2023). Study on physiological and biochemical parameters of sacha inchi leaf (Plukenetia volubilis L.) and the process of making tea bags in Vietnam. HNUE Journal of Science, Natural Sciences, 68 (3), 162-174. DOI: 10.18173/2354-1059.2023-0072
[30] Xiaoqiu S, Jinyu W, Shuaitao L & Yifei W, (2022). Formation of sacha inchi oil microemulsion systems: effects of non-ionic surfactants, short-chain alcohols, straight-chain esters, and essential oils. Journal of the Science of Food and Agriculture, 102 (9), 3572-3580. https://doi.org/10.1002/jsfa.11703.

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Published

25-06-2024