ANTIOXIDANT ACTIVITY AND SELECTED METABOLIC EFFECTS OF ETHANOL EXTRACT FROM MANGOSTEEN (Garcinia mangostana L.) PERICARP IN HIGH-FAT DIET–INDUCED MICE: AN IN VITRO AND IN VIVO STUDY
DOI:
https://doi.org/10.18173/2354-1059.2026-0011Keywords:
Garcinia mangostana, mangosteen pericarp, polyphenols, antioxidantactivity, high-fat dietAbstract
Mangosteen (Garcinia mangostana L.) pericarp is a rich source of polyphenolic xanthones and other bioactive compounds with potential antioxidant and metabolic regulatory properties. This study investigated the in vitro antioxidant activity and selected in vivo metabolic effects of ethanol extract from mangosteen pericarp. Mangosteen pericarp collected from Lam Dong province was extracted with 60% ethanol. The extract was characterized for total phenolic content by the Folin–Ciocalteu method. Antioxidant capacity was evaluated using the DPPH radical scavenging assay. For the in vivo study, male white mice (Mus musculus) were divided into four groups: a regular diet (RD), a high-fat diet (HFD), a HFD with extract at 300 mg/kg, and a HFD with extract at 400 mg/kg. Liver weight, spleen weight, and fasting blood glucose levels were measured after 8 weeks. The extract exhibited a high total phenolic content (200.16 ± 17.71 mg gallic acid equivalents/g extract) and showed dose-dependent DPPH radical scavenging activity with a half maximal inhibitory concentration (IC50) value of 118.87 ± 13.56 μg/mL, indicating a moderate but reproducible antioxidant capacity. In vivo, the HFD group showed significantly increased blood glucose levels (10.38 ± 3.54 mmol/L) compared to RD controls (7.57 ± 3.45 mmol/L). Treatment with the extract at 400 mg/kg significantly reduced glucose levels (5.16 ± 0.93 mmol/L, p < 0.05) compared to the HFD group. The extract at 300 mg/kg showed intermediate effects (9.89 ± 2.77 mmol/L). No statistically significant differences were observed in liver or spleen weights among the groups. In summary, the mangosteen pericarp ethanol extract is rich in phenolics, exhibits moderate antioxidant activity in vitro, and demonstrates dose-dependent protective effects against HFD-induced hyperglycemia. While the in vivo metabolic assessment was limited to selected endpoints, these findings provide preliminary evidence supporting the potential of the mangosteen pericarp extract for further investigation in diet-induced metabolic dysfunction models.
References
[1] Alberti KG, Eckel RH, Grundy SM, Zimmet PZ, Cleeman JI, Donato KA, Fruchart J-C, James WPT, Loria CM, Smith SC Jr, (2009). Harmonizing the metabolic syndrome: a joint interim statement of the international diabetes federation task force on epidemiology and prevention; National Heart, Lung, and Blood Institute; American Heart Association; World Heart Federation; International Atherosclerosis Society; and International Association for the Study of obesity. Circulation, 120(16), 1640-1645. doi: 10.1161/CIRCULATIONAHA.109.192644.
[2] Saklayen MG, (2018). The global epidemic of the metabolic syndrome. Current Hypertension Reports, 20(2), 1-8. doi: 10.1007/s11906-018-0812-z.
[3] Hariri N & Thibault L, (2010). High-fat diet-induced obesity in animal models. Nutrition Research Reviews, 23(2), 270-299. doi: 10.1017/S0954422410000168.
[4] Matsuda M & Shimomura I, (2013). Increased oxidative stress in obesity: implications for metabolic syndrome, diabetes, hypertension, dyslipidemia, atherosclerosis, and cancer. Obesity Research & Clinical Practice, 7(5), e330-e341.doi: 10.1016/j.orcp.2013.05.004.
[5] Newsholme P, Cruzat VF, Keane KN, Carlessi R & de Bittencourt Jr PIH, (2016). Molecular mechanisms of ROS production and oxidative stress in diabetes. Biochemical Journal, 473(24), 4527-4550. doi: 10.1042/BCJ20160503C.
[6] Newman DJ & Cragg GM, (2020). Natural products as sources of new drugs over the nearly four decades from 01/1981 to 09/2019. Journal of Natural Products, 83(3), 770-803. doi: 10.1021/acs.jnatprod.9b01285.
[7] Panche AN, Diwan AD & Chandra SR, (2016). Flavonoids: an overview. Journal of Nutritional Science, 5, e47. doi: 10.1017/jns.2016.41.
[8] Kedare SB & Singh RP, (2011). Genesis and development of DPPH method of antioxidant assay. Journal of Food Science and Technology, 48(4), 412-422. doi:10.1007/s13197-011-0251-1.
[9] Pedraza-Chaverri J, Cárdenas-Rodríguez N, Orozco-Ibarra M & Pérez-Rojas JM, (2008). Medicinal properties of mangosteen (Garcinia mangostana). Food and Chemical Toxicology, 46(10), 3227-3239. doi: 10.1016/j.fct.2008.07.024.
[10] Ovalle-Magallanes B, Eugenio-Pérez D & Pedraza-Chaverri J, (2017). Medicinal properties of mangosteen (Garcinia mangostana L.): A comprehensive update. Food and Chemical Toxicology, 109, 102-122. doi: 10.1016/j.fct.2017.08.021.
[11] Ibrahim MY, Hashim NM, Mariod AA, Mohan S, Abdulla MA, Abdelwahab SI & Arbab IA, (2016). α-Mangostin from Garcinia mangostana Linn.: An updated review of its pharmacological properties. Arabian Journal of Chemistry, 9(3), 317-329. doi: 10.1016/j.arabjc.2014.02.011.
[12] Akao Y, Nakagawa Y, Iinuma M & Nozawa Y, (2008). Anti-cancer effects of xanthones from pericarps of mangosteen. International Journal of Molecular Sciences, 9(3), 355-370. doi: 10.3390/ijms9030355.
[13] Jung HA, Su BN, Keller WJ, Mehta RG & Kinghorn AD, (2006). Antioxidant xanthones from the pericarp of Garcinia mangostana (Mangosteen). Journal of Agricultural and Food Chemistry, 54(6), 2077-2082. doi: 10.1021/jf052649z.
[14] Oh Y, Do HTT, Kim S, Kim YM, Chin YW & Cho J, (2020). Memory-enhancing effects of mangosteen pericarp water extract through antioxidative neuroprotection and anti-apoptotic action. Antioxidants, 10(1), 34. doi: 10.3390/antiox10010034.
[15] Zarena AS & Sankar KU, (2009). A study of antioxidant properties from Garcinia mangostana L. pericarp extract. Acta Scientiarum Polonorum Technologia Alimentaria, 8(1), 23-34.
[16] Noce A, Di Lauro M, Di Daniele F, Pietroboni Zaitseva A, Marrone G, Borboni P & Di Daniele N, (2021). Natural bioactive compounds useful in clinical management of metabolic syndrome. Nutrients, 13(2), 630. doi: 10.3390/nu13020630.
[17] Singleton VL, & Rossi Jr JA, (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16(3), 144-158. doi: 10.5344/ajev.1965.16.3.144
[18] Ipsen DH, Lykkesfeldt J & Tveden-Nyborg P, (2018). Molecular mechanisms of hepatic lipid accumulation in non-alcoholic fatty liver disease. Cellular and Molecular Life Sciences, 75(18), 3313-3327. doi: 10.1007/s00018-018-2860-6.
[19] Asghar A, Akhtar T, Batool T, Khawar MB, Nadeem S, Mehmood R & Sheikh N,(2021). High-fat diet-induced splenic, hepatic, and skeletal muscle architecture damage: cellular and molecular players. Molecular and Cellular Biochemistry, 476(10), 3671-3679. doi: 10.1007/s11010-021-04190-6.
[20] Fatoumata BA, Mohamet SENE & El Hadji Makhtar BA, (2020). Antidiabetic properties of Moringa oleifera: A review of the literature. Journal of Diabetes and Endocrinology, 11(1), 18-29. doi: 10.5897/JDE2019.0136.
[21] Deguchi Y & Miyazaki K, (2010). Anti-hyperglycemic and anti-hyperlipidemic effects of guava leaf extract. Nutrition & Metabolism, 7(1), 9. doi: 10.1186/1743-7075-7-9.
[22] Nguyen TH & Nguyen THH (2025). Antimicrobial, cytotoxic, and enzyme inhibitory activities of Garcinia mangostana L. pericarp extract relevant to diabetes and Alzheimer’s disease: Findings from an in vitro study. TNU Journal of Science and Technology, 230(13), 478-485. doi:10.34238/tnu-jst.12780.
[23] Choi YH, Bae JK, Chae HS, Kim YM, Sreymom Y, Han L & et al, (2015). α-Mangostin regulates hepatic steatosis and obesity through SirT1-AMPK and PPARγ pathways in high-fat diet-induced obese mice. Journal of Agricultural and Food Chemistry, 63(38), 8399-8406. doi: 10.1021/acs.jafc.5b01637.
[24] Chatatikun M, Tedasen A, Phinyo P, Wongyikul P, Klangbud WK, Kawakami F &et al., (2024). Hypoglycemic activity of Garcinia mangostana L. extracts on diabetes rodent models: A systematic review and network meta-analysis. Frontiers in Pharmacology, 15, 1472419. doi: 10.3389/fphar.2024.1472419.
[25] Evans JL, Goldfine ID, Maddux BA & Grodsky GM, (2002). Oxidative stress and stress-activated signaling pathways: A unifying hypothesis of type 2 diabetes. Endocrine Reviews, 23(5), 599-622. doi: 10.1210/er.2001-0039.
[26] Majdalawieh AF, Khatib BK & Terro TM, (2025). α-Mangostin is a xanthone derivative from mangosteen with potent immunomodulatory and anti-inflammatory properties. Biomolecules, 15(5), 681. doi: 10.3390/biom15050681.
